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  4. Rat growth hormone,GH ELISA KIT

Rat growth hormone,GH ELISA KIT

  • 中文名称：
    大鼠生长激素(GH)酶联免疫试剂盒
  • 货号：
    CSB-E07342r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：
    大鼠生长激素(GH)酶联免疫试剂盒（CSB-E07342r）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的GH1含量。GH1 即生长激素 1 基因，其编码的生长激素是调节生长发育的关键激素。研究发现，GH1 可促进细胞增殖、分化，影响蛋白质合成、脂肪代谢等。异常表达或功能缺陷会引发生长发育异常，如侏儒症、巨人症等，对它的研究有助于相关疾病诊疗。试剂盒检测范围为3.12 pg/mL-200 pg/mL，适用于体外实验中对大鼠模型相关生物样本的 GH 水平监测，如探究生长调节机制、评估药物干预对激素分泌的影响或研究代谢相关疾病的分子机制等科研领域，为动物实验提供可靠的检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Ghrh ELISA Kit; Somatoliberin ELISA Kit; Growth hormone-releasing factor ELISA Kit; GRF ELISA Kit; Growth hormone-releasing hormone ELISA Kit; GHRH ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    3.12 pg/mL-200 pg/mL
  • 灵敏度：
    0.78 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat GH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:100 Average % 98  
    Range % 94-105  
    1:200 Average % 96  
    Range % 90-102  
    1:400 Average % 100  
    Range % 94-106  
    1:800 Average % 93  
    Range % 89-97  
  • 回收率：
    The recovery of rat GH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 87-98  
    EDTA plasma (n=4) 96 91-101  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    200 2.718 2.801 2.760 2.580  
    100 2.346 2.405 2.376 2.196  
    50 1.754 1.763 1.759 1.579  
    25 1.126 1.157 1.142 0.962  
    12.5 0.657 0.668 0.663 0.483  
    6.25 0.458 0.467 0.463 0.283  
    3.12 0.341 0.358 0.350 0.170  
    0 0.179 0.181 0.180 　  
  • 本试剂盒所含材料：
      • A micro ELISA plate --The 96-well plate has been pre-coated with an anti-rat GH antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
      • Two vials lyophilized standard --Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • One vial Biotin-labeled GH antibody (100 x concentrate) (120 μl/bottle) --Act as the detection antibody.
      • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • One vial Biotin-antibody Diluent (15 ml/bottle) --Dilute the high concentration Biotin-antibody to an appropriate working solution.
      • One vial HRP-avidin Diluent (15 ml/bottle) --Dilute the high concentration HRP-avidin solution to an appropriate solution.
      • One vial Sample Diluent (50 ml/bottle)--Dilute the sample to an appropriate concentration.
      • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
      • One vial TMB Substrate (10 ml/bottle) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • One vial Stop Solution (10 ml/bottle) --Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --Cover the microplate when incubation.
      • An instruction manual

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  • 本试剂盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
  • 基因功能参考文献：
    1. IGF1 exerts a negative feedback on GH secretion by a transcriptional mechanism. PMID: 29080043
    2. The results suggest that GH regulates energy metabolism directly in myocytes and that UCP2 participates in the signal transduction pathway that functions downstream of the GHR/JAK/STAT. PMID: 27150070
    3. Impaired growth hormone-mediated signaling is observed in ethanol-exposed hepatocytes and is explained by differential effects of alcohol dehydrogenase (ADH)- and cytochrome P450 2E1 (CYP2E1)-mediated ethanol metabolism on the Jak2/STAT5B pathway. PMID: 28864499
    4. l-Ornithine stimulates growth hormone secretion through the sympathetic nervous and ghrelin systems. PMID: 28513740
    5. This study evaluated the role of growth hormone in the response to exogenous epidermal growth factor. PMID: 28223314
    6. This study indicates that the depressive-like behavior may be related to the decrease of Gh1 expression in the cerebellum and prefrontal cortex. PMID: 25716693
    7. estrogen deficiency impairs the induction of thyroid hormone activating enzyme D1 in the pituitary, and GH release by acute exercise. Also, acute D1 activation is essential for exercise-induced GH response PMID: 25874614
    8. constitutive SSTR3 activity mediates transcriptional repression of GH. PMID: 24606125
    9. GH serum concentration fell, with a modification of GH daily pattern after mealtime PMID: 24617825
    10. Linear growth impairment in chronic kidney disease can in part be explained by impaired long bone growth plate growth hormone receptor signaling through the JAK2/STAT5 pathway. PMID: 23715123
    11. A novel missense substitution single nucleotide polymorphism identified in the GH1 gene of Wistar-Imamichi rats. PMID: 24338415
    12. Data from mutant strain of rats suggest that ghrelin (Ghrl) participates in neurogenesis in adult hippocampus and leads to enhancement of cognitive function in mechanisms that are independent of growth hormone and insulin-like growth factor-1 (Igf1). PMID: 23774069
    13. This study compared the effect of a single IV bolus of a related molecule developed for clinical studies SXN101959 with a SC infusion of the somatostatin analog octreotide SMS201-995 to lower GH/IGF-I activity in growing male rats. PMID: 24029240
    14. CR-associated remodeling of WAT, which involves SREBP-1-mediated transcriptional activation and suppression of macrophage infiltration, is regulated in a GH-IGF-1-independent manner. PMID: 22645024
    15. GH increased the levels of glial fibrillary acidic protein (GFAP) and decreased the levels of vimentin. PMID: 23792323
    16. Data show alterations in growth hormone (GH) synthesis and expression of GH-releasing hormone receptor (GHRH receptor) on cells of the immune system that may play a role in the immune response in aging. PMID: 23770714
    17. Data indicate a putative hypoxia response element (HRE) in the growth hormone (GH) promoter at the region -176 bp to -172 bp that contains a copy of the hypoxia-inducible factor-1 (Hif-1) binding motif (5'-ACGTG-3'). PMID: 23639351
    18. an important role of growth cartilage GH/IGF-I axis regulation in a rat model of catch-up growth. PMID: 22583947
    19. Combinatorial interplay among multiple Stat5b-binding response elements with distinguishable biochemical properties is responsible for highly regulated control of IGF-I gene activity by growth hormone. PMID: 23185594
    20. in the absence of pituitary growth hormone the postnatal proliferation of cholinergic synapses in the rat spinal cord where AChE activity is abundant, is markedly reduced PMID: 22922167
    21. Chronic hypoxia induced decreases in serum growth hormone/Igf1 may be slightly attenuated by exercise/docosahexaenoic acid supplementation. PMID: 22865430
    22. analysis of enhanced oxidative stress in GH-transgenic rat PMID: 22370764
    23. GH/IGF-1 deficiency rendered the cardiovascular system more vulnerable to the deleterious effects of obesity. Lewis dwarf rats had a relative increase in blood glucose levels, lower insulin, and impaired glucose tolerance as compared with controls. PMID: 22080499
    24. The inhibitory effects of EGF on GH expression are mediated by MAPK activation in these cells. PMID: 22026435
    25. Data suggest that inductive/repressive effects of GH on hepatic P450 enzymes (i.e., steroid hydroxylases) are all exerted at transcriptional level; inductive/repressive effects of continuous presence of GH are direct effects on hepatocytes. [REVIEW] PMID: 22217849
    26. the inhibition of Stat5b expression in chondrocytes prevented the GH promoting effects on NF-kappaB-DNA binding, whereas the inhibition of NF-kappaB p65 expression or activity prevented the GH-dependent activation of IGF-1 and bmp2 expression. PMID: 21592969
    27. involvement of the GH/IGF-1 axis in the effect of CR for stress response, even if CR does not act solely through the GH axis. PMID: 21291903
    28. Growth hormone gene expression rises in the pituitary, while its receptor mRNA level in the hypothalamus decreases. PMID: 20814072
    29. GH stimulates recruitment of Stat5b to multiple dispersed regions within the IGFI locus PMID: 20378540
    30. This study investigated the: eEF1A and GH mRNA binding to cytoskeleton plus GH mRNA translation rate and GH secretion, in sham-operated and thyroidectomized rats treated with triiodothyronine or saline. PMID: 20015464
    31. data show that growth hormone is specifically imported in mitochondria, where it operates a direct metabolic effect, independently of cell surface receptors and signal transduction PMID: 20016135
    32. Bcl6 and STAT5 binding are inversely coordinated by the endogenous pulses of pituitary GH release, suggesting this male-specific transcriptional repressor modulates hepatic GH signaling to select STAT5 target genes PMID: 19797429
    33. GH and its receptor have roles in injury-induced neurogenesis PMID: 19524466
    34. evaluate the influence of GH deficiency on the skin nature, age-related changes in the dorsal skin histology were compared between male Mini and Wistar rats PMID: 11962746
    35. gene expression of GH and GHR mRNA in isolated oocytes and follicular wall cells of pre-antral follicles; localisation of immunoreactive IGF-I, IGFR, GH and GHR proteins in ovarian sections of 10-day-old rats PMID: 11964096
    36. role for GH signaling in the pathogenesis of early diabetic renal changes PMID: 12086960
    37. GH administration desensitizes the JAK2/STAT5 pathway PMID: 12161450
    38. study demonstrates that leptin but not growth hormone or insulin-like growth factor-I is involved in the development of insulin resistance in growing rats as a result of excess energy intake in the form of dietary fat PMID: 12624434
    39. endogenous pituitary GH exerts a feedback action on stomach ghrelin homeostasis PMID: 14530511
    40. critical components of GH-activated IGF-I, transcriptional pathway are two adjacent Stat5 binding sites in the second intron of the IGF-I gene located within a conserved region previously found to undergo changes in chromatin structure after GH treatment PMID: 14532269
    41. With IGF-I, contributes to changes in bone density and male reproductive organs. PMID: 15223841
    42. GH plays an important role in establishing the sex-dependent differences in liver nuclear protein content in rats PMID: 15456855
    43. functional recycling of retained hormones is not shared by all anterior pituitary cell types PMID: 15480745
    44. B, non T-non B, and CD4+-CD8+ cells correlated negatively with plasma prolactin. Modifications in mean value and 24-h rhythmicity of plasma prolactin and GH levels are presumably involved in the effect of social isolation on immune responsiveness. PMID: 15545705
    45. AGRP does not play a significant role in the feedback regulation of the GH secretion PMID: 15582725
    46. GH and IGF-I necessary for reproduction and growth of offspring early in life and for maintaining cognition and preventing cartilage degeneration later in life. PMID: 15790724
    47. Data show that cholangiocytes respond to growth hormone with production and release of IGF1 that modulates cell proliferation by transduction pathways involving IGF1-R, IRS1/2 and both ERK and PI3-kinase. PMID: 16083987
    48. hypoxia (continually or intermittently), restraint, cold alone or in combination modulate pituitary GH and hepatic IGF-I PMID: 16139950
    49. insulin may be necessary for sensitization of cells to GH-induced ERK1/2 activation in rat cells PMID: 16272159
    50. These results indicate that maternal exercise significantly increases plasma levels of GH, IGF-I and IGFBP-3 in the late period of pregnancy but causes adverse effects on fetal growth. PMID: 16340176

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Somatotropin/prolactin family
  • 数据库链接：


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