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  4. Recombinant TrfA transcriptional repressor protein (trfA)

Recombinant TrfA transcriptional repressor protein (trfA)

  • 中文名称：
    大肠杆菌trfA重组蛋白
  • 货号：
    CSB-YP357176ENL
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大肠杆菌trfA重组蛋白
  • 货号：
    CSB-EP357176ENL
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大肠杆菌trfA重组蛋白
  • 货号：
    CSB-EP357176ENL-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大肠杆菌trfA重组蛋白
  • 货号：
    CSB-BP357176ENL
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大肠杆菌trfA重组蛋白
  • 货号：
    CSB-MP357176ENL
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    trfA
  • Uniprot No.：
  • 别名：
    trfA; Plasmid replication initiator protein TrfA
  • 种属：
    Escherichia coli
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-382
  • 氨基酸序列
    MNRTFDRKAY RQELIDAGFS AEDAETIASR TVMRAPRETF QSVGSMVQQA TAKIERDSVQ LAPPALPAPS AAVERSRRLE QEAAGLAKSM TIDTRGTMTT KKRKTAGEDL AKQVSEAKQA ALLKHTKQQI KEMQLSLFDI APWPDTMRAM PNDTARSALF TTRNKKIPRE ALQNKVIFHV NKDVKITYTG VELRADDDEL VWQQVLEYAK RTPIGEPITF TFYELCQDLG WSINGRYYTK AEECLSRLQA TAMGFTSDRV GHLESVSLLH RFRVLDRGKK TSRCQVLIDE EIVVLFAGDH YTKFIWEKYR KLSPTARRMF DYFSSHREPY PLKLETFRLM CGSDSTRVKK WREQVGEACE ELRGSGLVEH AWVNDDLVHC KR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Required for initiation of plasmid DNA replication, along with host-derived DnaA and other host proteins. Both forms of the protein are capable of initiating plasmid replication in a number of Gram-negative bacteria. Binds to 8 17-base pair repeat sequences (iterons) in the RK2 minimal replication origin (oriV), opening the origin of replication. oriV opening does not absolutely require the presence of nucleotides; formation of open complex is somewhat enhanced by ATP or ATP gamma S, while DnaA or HU is required for full opening.; Also involved in plasmid copy number control, promoting intermolecular coupling of protein bound iterons at oriV, which inhibits replication initiation.
  • 亚细胞定位：
    Cell inner membrane; Peripheral membrane protein. Note=Probably the site where plasmid replication is initiated.