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  4. Recombinant Escherichia coli ATP-dependent Clp protease proteolytic subunit (clpP)

Recombinant Escherichia coli ATP-dependent Clp protease proteolytic subunit (clpP)

  • 中文名称：
    Recombinant Escherichia coli ATP-dependent Clp protease proteolytic subunit(clpP),Yeast
  • 货号：
    CSB-YP358828ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Escherichia coli ATP-dependent Clp protease proteolytic subunit(clpP),Yeast
  • 货号：
    CSB-EP358828ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Escherichia coli ATP-dependent Clp protease proteolytic subunit(clpP),Yeast
  • 货号：
    CSB-EP358828ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Escherichia coli ATP-dependent Clp protease proteolytic subunit(clpP),Yeast
  • 货号：
    CSB-BP358828ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Escherichia coli ATP-dependent Clp protease proteolytic subunit(clpP),Yeast
  • 货号：
    CSB-MP358828ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    clpP
  • Uniprot No.：
  • 别名：
    clpP; lopP; b0437; JW0427ATP-dependent Clp protease proteolytic subunit; EC 3.4.21.92; Caseinolytic protease; Endopeptidase Clp; Heat shock protein F21.5; Protease Ti
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    15-207
  • 氨基酸序列
    ALVPMV IEQTSRGERS FDIYSRLLKE RVIFLTGQVE DHMANLIVAQ MLFLEAENPE KDIYLYINSP GGVITAGMSI YDTMQFIKPD VSTICMGQAA SMGAFLLTAG AKGKRFCLPN SRVMIHQPLG GYQGQATDIE IHAREILKVK GRMNELMALH TGQSLEQIER DTERDRFLSA PEAVEYGLVD SILTHRN
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins. May play the role of a master protease which is attracted to different substrates by different specificity factors such as ClpA or ClpX. Participates in the final steps of RseA-sigma-E degradation, liberating sigma-E to induce the extracytoplasmic-stress response. Degrades antitoxin MazE.
  • 基因功能参考文献：
    1. Antirestriction activity of the mercury resistance nonconjugative transposon Tn5053 is controlled by the protease ClpXP PMID: 25735133
    2. The results demonstrate that ClpXP targets the helicase-nuclease (HsdR) subunit of EcoKI during dsDNA translocation on circular DNA but not on linear DNA. PMID: 25260590
    3. s found that adoption of a beta-hairpin loop at the N-terminal and the integrity of the hydrophobic cluster at the base of this loop are necessary elements for the axial gate to efficiently translocate protein substrates. PMID: 23927726
    4. These results demonstrate that ClpP allosterically impacts the polypeptide translocation activity of ClpA by reducing the cooperativity between ATP binding sites. PMID: 23639359
    5. When ClpX encounters a folded protein, it either overcomes this mechanical barrier or slips on the polypeptide before making another unfolding attempt. Binding of ClpP decreases the slip probability and enhances the unfolding efficiency of ClpX. PMID: 21529717
    6. data suggest that ClpAP and ClpXP may recognize and bind substrates in significantly different ways PMID: 15591068
    7. the main ATP-dependent proteases Lon and ClpP participate in the physiological disintegration of cytoplasmic inclusion bodies, their absence minimizing the protein removal up to 40% PMID: 15967532
    8. specific peptide sequences can function cooperatively to facilitate binding and unfolding by ClpA and degradation by ClpAP PMID: 16410355
    9. the ClpAP-specific adaptor, ClpS, is essential for degradation of N-end rule substrates by ClpAP in bacteria PMID: 16467841
    10. ClpXP is a critical factor in the cellular clearance of cytoplasmic RecN aggregates from the cell and therefore plays an important role in DNA damage tolerance PMID: 16914543
    11. Discrete static and dynamic interactions mediate binding and communication between ClpX and ClpP. PMID: 17612489
    12. ClpS functions, at least in part, as an allosteric effector of ClpAP, broadening understanding of how AAA+ adaptors control substrate selection. PMID: 18297088
    13. In the absence of ClpA, deletion of the ClpP N-terminus increases the initial degradation rate of large peptide substrates 5-15-fold. PMID: 18816064
    14. Loss of clpP and clpX genes leads to a moderate fourfold increase in spontaneous mutagenesis. PMID: 19040636
    15. ClpXP degrades UvrA at different rates during the distinct stages of post-UV recovery when the cells contain different levels of unrepaired cyclobutane pyrimidine dimers. PMID: 19183285
    16. analysis of ClpXP adaptors and substrates PMID: 19549779

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Peptidase S14 family
  • 数据库链接：

    KEGG: ecj:JW0427

    STRING: 316385.ECDH10B_0393