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  4. Rat α2 macroglobulin,α2 MG ELISA Kit

Rat α2 macroglobulin,α2 MG ELISA Kit

  • 中文名称：
    大鼠α2巨球蛋白(α2-MG)酶联免疫试剂盒
  • 货号：
    CSB-E13863r
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：
    大鼠α2巨球蛋白(α2-MG)酶联免疫试剂盒（CSB-E13863r）为竞争法ELISA试剂盒，定量检测血清、血浆样本中的A2M含量。A2M（α-2-巨球蛋白）是一种在血浆中含量丰富的糖蛋白，具有蛋白酶抑制作用，参与急性炎症反应。其研究机制涉及与多种免疫炎症因子结合，调节体内小分子平衡。在神经系统疾病中，A2M与阿尔茨海默病、多发性硬化等疾病相关，可能通过空间捕获蛋白酶来抑制其活性。试剂盒检测范围为3.13 ng/mL-800 ng/mL，该产品专为科研领域设计，可应用于炎症机制研究、肝肾功能评估、疾病动物模型分析及药物干预效果评价等方向，为探索α2 - MG在病理生理过程中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    A2m ELISA kit; A2m1Alpha-2-macroglobulin ELISA kit; Alpha-2-M ELISA kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma
  • 检测范围：
    3.13 ng/mL-800 ng/mL
  • 灵敏度：
    3.125 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat α2 MG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1000 Average % 97
    Range % 88-105
    1:2000 Average % 91
    Range % 89-102
    1:4000 Average % 93
    Range % 90-98
    1:8000 Average % 94
    Range % 87-98
  • 回收率：
    The recovery of rat α2 MG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 97 92-102
    EDTA plasma (n=4) 95 90-99
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average
    800 0.218 0.214 0.216
    200 0.451 0.461 0.456
    50 0.850 0.861 0.856
    12.5 1.225 1.242 1.234
    3.125 1.726 1.737 1.732
    0 1.944 1.967 1.956
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 功能：
    Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Protease inhibitor I39 (alpha-2-macroglobulin) family
  • 组织特异性：
    Highest constitutive expression in ovary. Low level in testis, uterus and non-acute phase liver. Protein found in plasma.
  • 数据库链接：


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